Abstract
The newborn mouse is relatively immuno-incompetent and potentially susceptible to tolerance to allogeneic cells. As a prelude to assessing the effects of monoclonal antibody (MoAb) on newborn immune function, we evaluated the kinetics of clearance of Thy1.2 positive cells following the injection of 1 day old C57BL/6 mice with anti-Thy1.2 MoAb (30-H12).
The percentage (mean ± SD) of splenocytes staining with Thy1.2 at 2, 7 and 14 days following the injection was significantly lower in both adults and newborns compared to control animals:
The rate of clearance in newborn mice was slower and less efficient than in adults. Changes in the percent of L3T4+ and LyT2+ cells indicated little modulation. Anti-Thy antibody was detected in the serum of newborn mice 7 days after injection and 2 days after injection in adults. Results indicate that the newborn C57BL/6 mouse can clear antibody-coated cells from the spleen and may be an appropriate model for the study of selective immunomodulation and tolerance induction.
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Culver, K., Wofsy, D., Smith, W. et al. IN VIVO T CELL DEPLETION IN NEWBORN MICE TREATED WITH ANTI-THY MONOCLONAL ANTIBODY. Pediatr Res 21 (Suppl 4), 310 (1987). https://doi.org/10.1203/00006450-198704010-00858
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DOI: https://doi.org/10.1203/00006450-198704010-00858