Abstract
Free oxygen radicals(superoxide anion, O2−) cause tissue damage in reperfusion injury of the intestine. The protective effect of a specific scavenger of O2− SOD, on weanling rabbit ileum during ischemia and reperfusion was evaluated. Twenty-three anesthetized weanling rabbits underwent laparotomy. The ileum was divided into 4 loops, each 8-10cm in length. Ischemia was induced in 2 loops by clamping the artery to the loop for 5mins; undamped loops served as controls. Eleven rabbits were pretreated with parenteral human SOD(5-10mg/kg, SC) at 0 and 16hrs. and surgery begun at 20hrs. Twelve received intraluminal SOD(10mg/kg) in 2 loops, and no SOD in 2 control loops. Animals were sacrificed 4 hrs. post surgery. Loops were fixed in formalin and examined histologically for the degree of mucosal necrosis by a pediatric pathologist who was blind to the conditions of the experiment. All rabbits given SC SOD had detectable serum levels of hSOD at 20 & 24 hrs. (.9-6.Omcg/ml).
Reperfused ileal loops are protected from mucosal necrosis by both parenteral and intraluminal SOD. This may be an important etiology and therapeutic modality in NEC.
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Vohra, K., Singh, I., Anderson, V. et al. ISCHEMIC INJURY TO NEWBORN RABBIT ILEUM: PROTECTIVE ROLE OF SUPEROXIDE DISMUTFISE (SOD). Pediatr Res 21 (Suppl 4), 281 (1987). https://doi.org/10.1203/00006450-198704010-00682
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DOI: https://doi.org/10.1203/00006450-198704010-00682