Abstract
ABSTRACT: Burst-promoting activity (BPA) produced by phytohemagglutinin(PHA)-stimulated cord blood mononuclear cells (MNC) was examined using the two-stage cell culture assays. Burst-promoting activity was measured as the increase in the number of early erythroid progenitor cells in 2-day incubation of peripheral blood MNC with or without the conditioned medium of PHA-stimulated MNC (PHA-LCM). Burst-promoting activity in PHA-LCM of cord blood was significantly lower than that of adult blood (37 ± 13 versus 105 ± 19%, mean ± SD, p < 0.01). No elevation of inhibitors to the erythroid colony growth was noted in PHA-LCM of cord blood. In contrary, the response of cord blood MNC to PHA was similar to that of adult blood MNC, as determined by colony-stimulating activity production and cell proliferation. These results showed that burst-promoting activity production by PHAstimulated MNC of cord blood was lower than that of adult blood.
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Iwai, Y., Miyanomae, T., Tanaka, M. et al. Low Burst Promoting Activity Production by Phytohemagglutinin-Stimulated Mononuclear Cells of Cord Blood. Pediatr Res 21, 285–288 (1987). https://doi.org/10.1203/00006450-198703000-00017
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DOI: https://doi.org/10.1203/00006450-198703000-00017