Abstract
ABSTRACT. Peptic-tryptic-cotazym and peptic-tryptic digests wore obtained, simulating in vivo protein digestion, from pure “bread” wheat gliadins and from rye, barley, and oats prolamine and tested on small intestine cultures from fetal rats. When tested at a concentration of 0.1 mg of peptides/ml of culture medium the peptic-tryptic-cotazym and peptic-tryptic digests of gliadin and prolamines were very active in slowing in vitro development of fetal rat intestine and in increasing the occurrence and severity of degenerative changes. The ability of some sugars to interfere with inhibition of fetal intestinal morphogenesis induced by these peptides was also tested. Mannan at a concentration of 0.1 mM was effective in allowing intestinal morphogenesis to take place in the presence of prolamine peptic-tryptic-cotazym and prolamine peptic-tryptic digests of the four toxic cereals. Some oligomers of N-acetylglucosamine were also effective in blocking the inhibitory effect of “bread” wheat gliadin peptides. These data are compatible with the hypothesis that some sugars may exert a protective effect on the toxic activity of cereal prolamin peptides on the human celiac intestine.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Auricchio, S., De Ritis, G., De Vincenzi, M. et al. Prevention by Mannan and other Sugars of in Vitro Damage of Rat Fetal Small Intestine Induced by Cereal Prolamin Peptides Toxic for Human Celiac Intestine. Pediatr Res 22, 703–707 (1987). https://doi.org/10.1203/00006450-198712000-00019
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1203/00006450-198712000-00019
This article is cited by
-
Binding of gliadin to lymphoblastoid, myeloid and epithelial cell lines
Folia Microbiologica (1995)