Abstract
In vitro alterations of chemotaxis in peripheral blood leukocytes from children with J.R.A. have been reported, and the scarce results are controversial. Chemotaxis of peripheral blood leukocytes from 15 children affected by J.R.A., aged from 3 to 10 years, (8 males and 7 females) were studied: the control group was constituted by children of the same age range, affected by orthopaedic diseases (without any inflammatory or metabolic disease). A modification of Boyden's technique was used, which works with a two compartment acrylic chamber, separated by a filtrating membrane with micropores. The chemotactic factor employed was the Escherichia coli lipopolysacharid (LPS). After the collection and preparation of leukocytes, the lower compartment of the acrylic chamber was filled with activated serum from a patient with J.R.A. or a control person. After one-hour incubation at 37%C, the filtrating membrane was adequately fixed and stained. It was observed no difference between the spontaneous and stimulated migration of leukocytes from patients with J.R.A. and controls. The chemotactic capacity of the activated serum of patients with J.R.A. over leukocytes of patients with J.R.A. and controls was lower than the activated serum of controls suggesting the existence of unknown serum factors, responsible for the inhibition of cell migration.
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Hilário, M., Sole, D., Goldenberg, J. et al. CHEMOTAXIS OF PERIPHERAL BLOOD LEUKOCYTES FROM PATIENTS WITH JUVENILE REUMATHOID ARTHRITIS (J.R.A.). Pediatr Res 22, 373 (1987). https://doi.org/10.1203/00006450-198709000-00054
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DOI: https://doi.org/10.1203/00006450-198709000-00054