Abstract
There is indirect evidence from clinical studies in human as well as in animal studies that infection and vaccination with B. pertussis might affect the β-adrenoceptor/cyclic AMP system. In order to study whether this view warks, the adrenergic system was investigated on lymphocytes in children within the paroxysmal stage of P, in children after vaccination (V.) with P-mono-vaccine, and in an age-matched control group.
Methods: β-adrenoceptor (β-R) density was estimated by means of binding studies using 125-J-cyanopindolol. In children with P β-E number of lymphocytes was 2-3 fold lower compared to controls and after V. High and low affinity states of the β-R, indicating the intrinsic activity, were obtained by Hofstee-plot-transformation of salbutanol displacement curves. Basal levels, as well as maximum cAMP accumulation after specific (isoprenaline = IPN) and unspecific (forskoline = FO) β-R stimulation, were determined using a protein binding assay.
Results: In children with P β-R number of lymphocytes was 2 to 3fold lower compared to controls. In addition, an increased number of low affinity state β-R indicated impaired function. Decreased cAMP levels in both, the vaccination children and those suffering from P were observed. However, the latter showed distinct reduction of IPN induced maximal cAMP accumulation.
It is concluded that P-infection, as well as V., strongly affects the adrenergic system.
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Griese, M., Reifenhäuser, A. & Reinhardt, D. IMPAIRED β-ADRENERGIC FUNCTION IN LYMPHOCYTES OF CHILDREN WITH PERTUSSIS (P). Pediatr Res 22, 240 (1987). https://doi.org/10.1203/00006450-198708000-00162
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DOI: https://doi.org/10.1203/00006450-198708000-00162
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