Abstract
Several methods have been introduced for the assessment of chemotactic activity of mononuclear and polymorphonuclear leucocytes /PMKs/. These techniques mostly base on the original assay of Boyden. We have recently devised a simple migration test modifying a commercially available Huclepore filter holder /EFH/ and filter membrane /pore diameter 5 μm, pore length 10 μm, Huclepore Corporation, Pleasanton, USA/. The upper compartment of the NFH is loaded with 100 μl of isolated human PMKs suspended in Hanks' balanced salt solution. The lower compartment contains 400 μl of the same suspension medium with or without chemoattractive agent. The loaded NFH is incubated at 37 C for 45 minutes. Then, the content of lower compartment is aspirated and the concentration of PMNs is counted in this suspension. Preliminary experiments show the usefulness of the method which is highly sensitive to distinguishing random and directed migrations /mean factor 3.2±0.5/. The coefficient of variation was between 12 and 18% using same suspensions.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bogar, L., Molnar, Z. & Tekeres, M. THE NUCLEPORE FILTER HOLDER: A NEW METHOD FOR CELL MIGRATION TEST. Pediatr Res 22, 220 (1987). https://doi.org/10.1203/00006450-198708000-00042
Issue Date:
DOI: https://doi.org/10.1203/00006450-198708000-00042