Abstract
Islet cell surface antibodies (ICSA) have been detected in insulin-dependent diabetes (IDD). Current techniques for the detection of ICSA often suffer from a lack of standardization and from a poorly defined specificity. We have developed a standardized method to quantify binding of circulating IgM and IgG fractions with the surface of purified rat pancreatic B cells; parallel experiments on islet non-B cells serve as control for islet cell specificity. Using this procedure no abnormalities were detected in the binding of circulating IgM from 30 recent onset IDD to islet B or non-B cells, comparison being made with 30 healthy age-matched controls. On the other hand, the IgG fraction from IDD patients was found to bind selectively to islet B cells (p 0.01) while no binding was observed with IgG from the normal controls. It is concluded that the developed procedure allows the recognition, quantification and specification of autoimmune reactions against surface components of islet cells.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vercammen, M., Balduck, N., Gorus, F. et al. Detection of surface antibodies against pancreatic B cells in insulin-dependent diabetes. Pediatr Res 22, 218 (1987). https://doi.org/10.1203/00006450-198708000-00029
Issue Date:
DOI: https://doi.org/10.1203/00006450-198708000-00029