Abstract
Both prostanoids and leukotrienes are metabolic products of arachidonate via separate enzymatic pathways. We have previously shown that prostaglandin E2 content in small intestinal mucosa from ovalbumin (OA) sensitized rats is elevated as compared to controls. The purpose of this investigation was to establish whether there is an elevated LTB4 content in the intestinal mucosa in rats suffering from an immediate type gastrointestinal allergic reaction. 8 rats of the Hooded-Lister strain were sensitized with 250 μg OA IP together with B. Pertussis adjuvant. On the 14th day a booster of 2.5 μg OA was given IP. 8 control rats were given adjuvant only. 5 days after the booster the test and control rats were challenged with 25 mg OA intragastrically and 45 minutes later the rats were sacrificed and 5 cm of proximal small intestine removed. LTB4 content of scraped mucosa was determined by radioimmnunoassay. It was found that the LTB4 content in the sensitized intestine was 50±9 pg/mg tissue as compared to a level of 38.7±8.8 pg/mg tissue in controls. This difference was significant (p< 0.025). We conclude that LTB4 may participate in intestinal immediate type responses and may explain some of the clinical manifestations of food protein allergy.
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Branski, D., Eran, M., Weidenfeld, J. et al. 73. LEUKOTRIENE B4 (LTB4) CONTENT OF SMALL INTESTINAL MUCOSA IN RAT MODEL OF PREOTEIN HYPERSENSITIVITY. Pediatr Res 22, 108 (1987). https://doi.org/10.1203/00006450-198707000-00094
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DOI: https://doi.org/10.1203/00006450-198707000-00094