Abstract
By using “in situ” hybridization methodology we have examined lymphocytes from peripheral blood from HBsAg positive and negative patients for the presence of HBV RNA. Mononuclear cells preparations were hybridized with a S35 or H3 labeled HBV specific DNA probe and exposed to autoradiographic emulsion.
42 subjects were studied (25 children, 17 adults). HBV RNA was present in the lymphocytes of 10/17 HBsAg+ HBeAg+, 8/18 HBsAg+ HBeAg-, 1/3 anti-HBs+ patients, 0/4 control subjects. The frequency of labelled cells varied from one patient to another from 1 to 10 %.
There is no difference between children and adult populations, and no correlation either the type of liver disease or presence of HBV DNA in the serum. These results demonstrate the presence of viral RNA In mononuclear cells and indicate that the DNA detected so far in human mononuclear cells is transcriptionnally active.
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Hadchouei, M., Pasquinelli, C., Scotto, J. et al. 63. DETECTION OF LYMPHOCYTES EXPRESSING HEPATITIS B VIRUS IN PERIPHERAL BLOOD FROM HBsAg POSITIVE AND NEGATIVE CHILDREN AND ADULTS BY “IN SITU” HYBRIDIZATION. Pediatr Res 22, 106 (1987). https://doi.org/10.1203/00006450-198707000-00084
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DOI: https://doi.org/10.1203/00006450-198707000-00084