Abstract
To compare in vivo and in vitro responses of fecal flora to L, breath was collected from 15 BR and 12 BO infants aged 0.1-6 mo and analyzed for H2 and CH4. Stools from 5 BR and 8 BO were analyzed for H2, lactic acid (LA), glucose (GL), and galactose (GA) before and after anaerobic incubation with 1.25% L at pH 6.8 and 5.5. Peak breath H2 values for BR and BO were 36±38 ppm (range 2-120) and 16±10 ppm (4-25). Breath CH4 values for BR and BO were 6±3 (0-12) and 8±3 (2-11).
* Preincubated stool; †Postincubation pH 6.8 and ‡5.5. Preliminary results indicate that stool incubates at pH 6.8 of BO infants produced more H2, GL, and GA than those of BR who accumulated more LA. At pH 5.5, H2 production decreased in both groups; GL and GA accumulation increased further in BO. This is the first report of CH4, production in infants. Fecal flora of BO appeared to have a limited capacity to utilize excessive amounts of GL and GA, particularly at pH 5.5, while BR continued to transform GL and GA into LA. We postulate that these differences may explain the means by which BR and BO metabolized malabsorbed L in vivo.
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Lifschitz, C., Chen, M. & Nichols, B. 23. IN VIVO AND IN VITRO RESPONSE TO LACTOSE (L) BY THE FLORA OF BREAST-(BR) AND BOTTLE-FED (BO) INFANTS. Pediatr Res 22, 100 (1987). https://doi.org/10.1203/00006450-198707000-00044
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DOI: https://doi.org/10.1203/00006450-198707000-00044