Abstract
To compare different aspects of postnatal MVM maturation, MVM were prepared from rats, starting from new-borns to the age of 12 weeks. MVM fluidity was studied by fluorescence polarization using different labels. Cholesterol and phospholipid composition of MVM was analysed. MVM binding studies were performed using radioiodinated probes. Membrane fluidity was increased in newborn MVM, compared to adults. There was a continuous decline of MVM fluidity throughout maturation. Changes in fluidity were accompanied by an increase of cholesterol/phospholipid molar ratio. Continuous maturational changes in membrane glycosylation wore indicated by differential specific binding of concanavalin A, soybean agglutinin, and peanut lectin. Contrary to the continuous MVM changes, food protein binding to MVM (cow's milk proteins, wheat gliadin peptides) was decreasing stepwise between 6-8 weeks of age. This was not related to weaning. It remains to be shown how different aspects of postnatal MVM maturation are interrelated, and how they are connected to the development of food protein tolerance or allergy in young individuals.
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Stern, M., Gellermann, B., Hübner, C. et al. 10. POSTNATAL MATURATION OF SMALL INTESTINAL MICROVILLUS MEMBRANES (MVM). Pediatr Res 22, 97 (1987). https://doi.org/10.1203/00006450-198707000-00031
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DOI: https://doi.org/10.1203/00006450-198707000-00031