Abstract
ABSTRACT. Neonatal mouse astroglial cells cultured in a serum-free medium synthesize and secrete a trophic growth factor which resembles nerve growth factor (NGF). The NGF-like factor reacts with antiserum to β subunit of NGF(β-NGF) and, after labeling with [35S] cystine, migrates similarly to purified mouse β-NGF in SDS polyacrylamide gel electrophoresis and Sephadex G-100 gel filtration systems. The astrocyte cell NGF-like factor displays β-NGF-like neurite growth-promoting activity for the clonal rat pheochromocytoma (PC-12) cell line and this bioactivity is blocked by β-NGF antiserum. These results indicate that NGF-like factor synthesized and secreted by astroglial cells, is similar, if not identical, to β- NGF from the mouse submandibular gland and further support a potential role for NGF in the central nervous system.
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Tarris, R., Weichsel, M. & Fisher, D. Synthesis and Secretion of a Nerve Growth- Stimulating Factor by Neonatal Mouse Astrocyte Cells in Vitro. Pediatr Res 20, 367–372 (1986). https://doi.org/10.1203/00006450-198604000-00022
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DOI: https://doi.org/10.1203/00006450-198604000-00022
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