Abstract
Cyst(e)ine metabolism is a source of intracellular oxidant stress(Astler et al, J Clin Invest 76:567, 1985). We studied the cyst(e)ine effect on platelet(plt)suspensions. Catalase activity of plts incubated 60 min at 37°0 with 100 μM cyst(e)ine, 1 mM 3-amino-1, 2, 4-triazole (AT), 0.2 mM DL-Buthionine-(S, R)-sulfoximine(inhibitor of glutathione cycle)and then washed 3 times, was measured polarographycally. Plt aggregation was performed by standard method. Catalase activity(determined by O2 generated/mg protein after H2O2 addition)was inhibited by about 50% after cysteine,and by about 30% after cystine addition (n=20, p<0.01). Cysteine inhibited by 40% pit aggregation, whereas cystine inhibited 2/3 of 20 samples. This effect was partially reversed by externally added catalase. The partial inhibition of catalase in the presence of AT indicates the production of H2O2 (AT inhibits catalase only in the presence of H2O2. Our data suggest that cyclic oxidation-reduction of thiols, of pharmacologic or physiologic interest, may affect glutathione-depleted plts, by producing H2O2.
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Menichelli, A., Del, P., Di, G. et al. 85 THE EFFECT OF CYST(E)INE ON HUMAN PLATELETS. Pediatr Res 20, 1048 (1986). https://doi.org/10.1203/00006450-198610000-00139
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DOI: https://doi.org/10.1203/00006450-198610000-00139