Abstract
ABSTRACT. An Escherichia coli derived somatomedin-C/IGF-I preparation (rec-IGF-I) with an amino acid sequence identical to the natural IGF-I derived from human plasma, increases body length and weight, as well as the growth of several organs of Snell dwarf mice, when administered for 4 wk. After 2 wk of treatment rec-IGF-I (22.2 μ/day) induced a significant increase over buffer treated controls, to a comparable degree as obtained with bacterially synthesized human growth hormone (bhGH; 8.4 μ/day). The weight/length ratio of rec-IGF-I and bhGH-treated dwarf mice after 4 wk of treatment were not significantly different. A significant increase over controls was obtained with both preparations. Organs with increased weights after bhGH treatment (brain; submandibular salivary glands; heart, liver, kidneys, thymus, and spleen) were also heavier after rec-IGF-I. Significance was only reached for the kidneys and the spleen and the musculus quadriceps femoris. Organs weights expressed as a percentage of body weight of bhGH and rec-IGF-I treated dwarfs were similar except for the relative weight of the heart of the bhGH group, which was significantly increased compared to the controls and the rec-IGF-I group. These data resolve the issue as to whether or not pure SM-C/IGF-I will induce growth in length and demonstrate the usefulness of recombinant IGF-I in the studies of growth regulation.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Buul-Offers, S., Ueda, I. & Van Den Brande, J. Biosynthetic Somatomedin C (SM-C/IGF-I) Increases the Length and Weight of Snell Dwarf Mice. Pediatr Res 20, 825–827 (1986). https://doi.org/10.1203/00006450-198609000-00002
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1203/00006450-198609000-00002
This article is cited by
-
Preclinical and Clinical In Vitro In Vivo Correlation of an hGH Dextran Microsphere Formulation
Pharmaceutical Research (2007)
-
Evidence for suppression of immune function by insulin-like growth factor-1 in dwarf rats in vivo
Experientia (1996)
-
The role of insulin-like growth factors and IGF-binding proteins in the physiological and pathological processes of the kidney
Virchows Archiv B Cell Pathology Including Molecular Pathology (1992)
-
Somatomedins: Chemical and functional characteristics of the different molecular forms
Journal of Endocrinological Investigation (1989)
-
Kidney tissue somatomedin C and initial renal growth in diabetic and uninephrectomised rats
Diabetologia (1988)