Abstract
The expression, biosynthesis and processing of SC were studied by continuous labelling as well as pulse/chase experiments using 33S-methionine in organ culture of human small intestinal biopsies and in a transformed human intestinal cell line, HT-29.
In both systems SC was first produced as a precursor molecule of Mr=100,000 which was converted to a Mr=110,000 form upon acquisition of peripheral carbohydrates. After 1 h of chase a Mr=80,000 species appeared which was then also found in the culture medium, i.e. was secreted. The appearance of this Mr=80,000 form was totally prevented by the addition to the culture medium of leupeptin, clearly showing it to be a proteolytic cleavage product. In cultured biopsies an additonal SC species was produced in the form of a doublet of Mr=60,000 and which was also secreted into the medium.
Conclusion: These results show that in human small intestinal epithelial cells SC is produced differently to other tissues.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sterchi, E., Naim, H., Buts, J. et al. EXPRESSION AND BIOGENESIS OF SECRETORY COMPONENT (SC). IN HUMAN SMALL INTESTINAL EPITHELIAL CELLS. Pediatr Res 20, 704 (1986). https://doi.org/10.1203/00006450-198607000-00111
Issue Date:
DOI: https://doi.org/10.1203/00006450-198607000-00111