Abstract
Cholera-like enterotoxin (CLT) production by campylobacter jejuni/coli (CJ) was shown in a new medium in which 25 strains of CJ isolated from diarrhoeic stools in children were grown for 24j at 42″. The presence of CLT was demonstrated by means of the Chinese Hamster Ovary Cells <CHP> elongation assay. (1). Though the majority of the strains produced CLT, the amount varied significantly from strain to strain. The morphological changes of CHO cells were neutralized by antisera against cholera-toxin (CT) and both porcin and human E.Coli LT.
In a second study, a new cytotoxic factor (CF) killing CHO cells (2), was shown in 24/24 stains of CJ from children with endoscopically and histoligically proven invasive colitis but also in 20/26 CJ strains from children with watery diarrhoea. Antiserum against shiga-toxin did not neutralize the CF.
Conclusions:
1) CLT produced by CJ is similar to CT and is produced in small amounts.
2) CF produced by CJ is different from shiga-toxin.
3) Role and importance of CLT and CF in overt intestinal CJ infection is to be further evaluated.
(1) Guerrant et al. Inf. & Inn., 10:326, 1974
(2) Goossens et al. Lancet i, 511, 1985
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Goossens, H., Boeck, M., Rodesch, P. et al. CHOLERA-LIKE TOXIN AND CYTOTOXIC FACTOR PRODUCTION BY CAMPYLOBACTER JEJUNI/COLI. Pediatr Res 20, 691 (1986). https://doi.org/10.1203/00006450-198607000-00036
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DOI: https://doi.org/10.1203/00006450-198607000-00036