Abstract
In situ hybridization and Southern blot analysis were performed on bone marrow from a child with Ph' positive 9; 22 ALL and on PA 682, a cell line from a patient with 8; 22 Burkitt's lymphoma (BL). We used a cDNA probe from the constant region of the immunoglobulin light chain gene complex (IGLC) to examine the translocation breakpoint in 22q11. In PA 682, the results of four “in situ” experiments with c-lambda reveal hybridization to the 8q+ and the normal 22 and no hybridization to the 22q-. These results suggest that in 8;22 BL, all of c-lambda of IGLC is translocated onto the 8q+ chromosome and the translocation breakpoint is proximal to c-lambda. We also performed two “in situ” experiments with the c-lambda probe on direct marrow preparations from a child with Ph' positive 9;22 ALL. In each experiment c-lambda is hybridized to the normal 22 and to the 22q- as well as to the 9q+. The levels of c-lambda hybridization to the 9q+, between 6-7.3% of the total grains, were significantly higher than background levels. These results suggest that the translocation breakpoint of 9;22 ALL interrupts the constant region cluster of IGLC and is distal to the 22q11 breakpoint of 8;22 BL.
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Cannizzaro, L., Nowell, P., Erikson, J. et al. 806 TRANSLOCATION BREAKPOINT MAPPING OF 9;22 ALL AND 806 8;22 BURKITT'S LYMPHOMA REVEALS DIFFERING BREAK-POINTS WITHIN 22q11. Pediatr Res 19, 245 (1985). https://doi.org/10.1203/00006450-198504000-00836
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DOI: https://doi.org/10.1203/00006450-198504000-00836