Abstract
Expression of polymorphic MHC class II antigens by accessory monocytic cells is required for the ability to present antigens to T cells and to regulate immune reactivity.In man it has been shown that HLA-D contains at least three well defined loci:DR,DS(DC)and SB.We demonstrate that fresh isolated human blood monocytes evaluated by two color FACS analysis with respect to the monocyte antigen M3 and the HLA-D determinants DC,DR may be divided into two major subpopulations: M3+ DR+ DC− and M3+ DR+DC+.The HL-60 promyelocitic cell line induced either by 1, 25(OH)2D3 or by PMA acquire phenotypically distinct surface markers with respect to M3 and DR,DC antigens.Indeed 1,25(OH)2D3 preferentially induce the expression of an M3+ DR+DC−monocyte population whereas PMA preferentially induce a population co-expressing the three antigens (M3+ DR+DC+).The evaluation of the ability of the induced cells to perform ADCC showed that 1,25(OH)2D3, in contrast to PMA, induce an increasing killing activity. These data suggest that 1,25(OH)2D3 and PMA induce the differentiation of phenotypically and functional different monocyte subpopulations. The evaluation of ADCC of cell sorted M3+DR+DC− 1,25(OH)2D3 induced HL-60, in comparison to the M3− population showed that the ADCC is mainly restricted to the M3+DR+DC− subset.
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Chini, L., Galli, E., Moschese, V. et al. HL-60 DIFFERENTIATION BY 1,25(OH)2D3 AND PMA. Pediatr Res 19, 1084 (1985). https://doi.org/10.1203/00006450-198510000-00098
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DOI: https://doi.org/10.1203/00006450-198510000-00098