Abstract
We reported (1) the isolation of an urate binding α2-globulin from human serum. Now we wish to determine the physiologic role of this urate binding α2-globulin in healthy subjects and gouty patients. Antibodies against purified α2-globulin were prepared by rabbit immunization. Several injections of Freund's incomplete adjuvant mixed with an aqueous solution of α2-globulin were repeated to increase immune response. Rabbit was bled by cardiac puncture. r-globulin fraction was separated from the serum by ammonium sulphate precipitation. Double immunodiffusion in agar was used for semiquantitative analysis of the rabbit serum immunoglobulines. The formation of a single precipitation line between the purified α2-globulin and its corrisponding antiserum can be utilized as a rough qualitative estimation of antibody purity. High purified antibodies were obtained from rabbit antiserum by affinity chromatography technique ( α2-globulin-Sepharose). These purified antibodies will be utilized to assay serum levels of α2-globulin in healthy subjects and gouty patients.
1. Appl.Biochem.Biotech.7,189,1982
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Mazzoni, M., Lucacchini, A., Bazzichi, L. et al. URATE BINDING α2–GLOBULIN: SPECIFIC ANTIBODY PREPARATION: 130. Pediatr Res 19, 765 (1985). https://doi.org/10.1203/00006450-198507000-00150
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DOI: https://doi.org/10.1203/00006450-198507000-00150