Abstract
Purine biosynthesis and growth rate studies were carried out on a mutant of Chinese Hamster Ovary Cells (CHO-Ade I) deficient in the adenylate biosynthetic enzyme Adenylosuccinase (ASase). The ratio of the rate of adenylate to guanylate synthesis are altered in CHO-Ade I (3:88) compared to the nearly equal distribution of these nucleotides (49:51) in the wild type parent line CHO-K1. The decrease in the ratio of adenylates to guanylates results from an apparent stimulation in the rate of guanylate synthesis in addition to the expected reduced rate of adenylate synthesis. In comparison to the wild type CHO cells the ASase deficient cells show an increased accumulation of hypoxanthine-containing compounds in both the cell and media (excreted) fractions. CHO-Ade I cells display a marked elevation in the activity of the branch point enzyme adenylosuccinate synthetase suggesting a direct regulation of this enzyme by either adenylosuccinate or the ultimate product adenylates. Similar to patients with a deficiency of this enzyme SAICA riboside and succinyladenosine are excreted into the medium by CHO-Ade I cells.
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Laikind, P., Gruber, H., Hoffer, M. et al. STUDIES OF PURINE BIOSYNTHESIS IN CHINESEHAMSTER CELL MDTANTS DEFICIENT IN ADENYLOSUCCINASE: 108. Pediatr Res 19, 761 (1985). https://doi.org/10.1203/00006450-198507000-00128
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DOI: https://doi.org/10.1203/00006450-198507000-00128