Abstract
A rapid and accurate means of determining the percentage of Hb S in blood would be of value in the management of sickle cell patients undergoing emergency exchange transfusions and on chronic transfusion programs. To determine the usefulness of the 2-hour SCP in predicting the % Hb S we compared the % of sickled cells in blood incubated for 2 hours in sodium metabisulfite to the % Hb S simultaneously determined by column chromatography (Sickle Cell Quik Column Method, Helena Laboratory.) Seventeen blood samples from 6 transfused patients with homozygous sickle cell disease were studied. Combined data from the 17 samples revealed a mean (±SEM) % of sickled cells determined by the 2-hour SCP of 30±6 and a mean % Hb S by column of 30±5. There was no significant difference between the two values as determined by the paired T-test (p=.96). Comparison of the two methods on individual samples revealed that 8 determinations by the 2-hour SCP were higher than those obtained by the column method, while 7 were lower. The discrepancy between the two methods on individual samples was usually less than 10 percentage points, although 6 samples had differences of 10-18 percentage points. We conclude that the 2-hour SCP is a simple, reliable, and accurate method of estimating the % Hb S and can be used as a guide in the management of sickle cell patients undergoing transfusion therapy.
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Ritchey, A., Sullivan, E., Rooks, Y. et al. 934 THE TWO-HOUR SICKLE CELL PREP (SCP) FOR RAPID DETERMINATION OF SICKLE HEMOGLOBIN (HB S). Pediatr Res 19, 266 (1985). https://doi.org/10.1203/00006450-198504000-00964
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DOI: https://doi.org/10.1203/00006450-198504000-00964