Abstract
The supernatants from human blood platelets activated by thrombin are chemotactic for polymorphonuclear cells(PMNs).We examined other functional and metabolic responses of PMNs to thrombin-activated vs unstimulated platelet supernatants.We showed that the incubation of PMNs with supernatant from activated platelets caused a significant increase in the rate of aggregation elicited by opsonized-zymosan(opZ), as compared to that from unstimulated platelets On the contrary,supernatants from activated platelets caused a 50% decrease in both the killing of staphylococcus aureus and in the luminol-enhanced chemiluminescence by PMNs.No influence on O2 consumption and O2 production by opZactivated PMNs was observed.Supernatants from activated platelets were able to reduce the release of myeloperoxidase from opZ-stimulated PMNs by 30 to 50%.The leakage of LDH from activated platelets was less than 3%,and no catalase and SOD activity was found in the supernatants.These data show that activated human platelets excrete some heat-stable factor(s),which modulate the PMN response to the stimuli.
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Chelli A, M., D, D., A, FA. et al. INTERACTION OF ACTIVATED HUMAN PLATELETS WITH POLYMORPHONUCLEAR CELLS. Pediatr Res 18, 807 (1984). https://doi.org/10.1203/00006450-198408000-00094
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DOI: https://doi.org/10.1203/00006450-198408000-00094