Abstract
Sarcolemmal damage and tissue Ca2+ uptake were determined during hypoxia and re-O2 in isolated, arterially perfused newborn (NB) and adult (A) rabbit hearts. Tissue 47Ca2+ uptake was measured by δ -probe. CK release from the tissue was determined in the venous effluent and was used as an indicator of the sarcolemmal damage. During 40 min of hypoxia, tissue Ca2+ was unchanged. CK release (IU/g dry wt) was observed only in the A. During re-O2, tissue Ca2+ increased [Δ Ca2+ (mmol/kg dry wt)] and CK release was observed, and these two values in the A were significantly greater than in the NB. Recovery of mechanical function [+dT/dt (max), % of control] during re-O2 in the NB was significantly greater than in the A.
These data suggest that tissue Ca2+ gain and/or sarcolemmal damage may be responsible for the impaired recovery of myocardial mechanical function after hypoxia.
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Nakanishi, T., Young, H., Shimizu, T. et al. 174 EFFECTS OF HYPOXIA AND REOXYGENATION (re-O2) ON CREATINE KINASE (CK) RELEASE AND TISSUE Ca2+ UPTAKE IN THE NEWBORN MYOCARDIUM. Pediatr Res 15 (Suppl 4), 468 (1981). https://doi.org/10.1203/00006450-198104001-00183
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DOI: https://doi.org/10.1203/00006450-198104001-00183