Abstract
Our goal was to help define host resistance to MCMV. MCMV killing of CBA, C3H, BALB/c and C57B16 weanlings was compared to MCMV multiplication in secondary MECC prepared from these mouse strains. We know that others have not found differences in MECC response to MCMV, and differences we saw were small. A 10% w/v salivary gland homogenate harvested from CD-1 weanling mice 3 wks after IP inoculation was utilized as MCMV. In a representative experiment 106 PFU MCMV IP killed none of the CBA and C3H mice, but 100% of BALB/c and 40% of C57′s. The number (av. of 10 plates) and size (av. of 50) of plaques produced on the various MECC were: CBA 77 plaques, av. size 0.3 mm; C3H 142, 0.3; C57B16 116, 0.5; BALB/c 150, 0.6. Several experiments comparing only CBA and BALB/c mice and MECC (the most and least resistant in vivo and in vitro) confirmed these results. MCMV growth was more (5 fold) in BALB/c than in CBA MECC and cytopathology was somewhat greater. The sensitivity of CBA and BALB/c MECC to IFN and an IFN inducer was then determined IFN (Cal Biochem) reduced MCMV growth slightly (3-10 fold) more in CBA than in BALB/c MECC. When Poly(I)·Poly(C) (P.L. Biochemicals) 10ug/dish was used to induce IFN, MCMV growth was reduced much more in CBA than in BALB/c MECC: 6 d post-inoc of 100 PFU, BALB/c had 55 × 103 PFU/ml and CBA 76 × 101. These results indicate that resistance to MCMV is conferred in part by an innate, probably genetically determined, non-immune cellular resistance which can be demonstrated by the capacity of fibroblast cultures to respond to IFN and an IFN inducer. (Supported by USPHS grant no. NS14763)
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Medearis, D., Kelly, T. 1046 INNATE, INTERFERON (IFN) MEDIATED, CELLULAR RESISTANCE TO MOUSE CYTOMEGALOVIRUS (MCMV) DEMONSTRATED IN EMBRYO FIBROBLAST CULTURES (MECC). Pediatr Res 15 (Suppl 4), 617 (1981). https://doi.org/10.1203/00006450-198104001-01072
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DOI: https://doi.org/10.1203/00006450-198104001-01072