Abstract
Mouse Leukemia L1210 is an extensively used model for cancer chemotherapy studies. An unique microwave irradiation exposure system was used to assay the effects of hyperthermia, microwave irradiation (MWR), and hydrocortisone succinate (HCS) on L1210 cells ability to form colonies in a methylcellulose culture system. MWR was given at 2450 MHz frequency with 1000 mW/cm2 power output for 30 min. L1210 cells were suspended in a medium and then exposed as follows: Sham at 37°C and 42°C; MWR at 37°C and 42°C. All 4 exposure sets were then plated at a cell concentration of 5,000 cells per plate either with no drug, or with 10−5M, 10−6M, 10−7M or 10−8M HCS per plate. At day 8 of culture the colony number was scored in each of the plates. Pre-exposure to MWR, hyperthermia or combination of the two had no effect on the cell's ability to form colonies. HCS when added to culture plates inhibited the colony formation in a dose-dependent fashion. However, no further inhibition was observed by pre-exposing cells to MWR or hyperthermia alone. A significant inhibition (50%) in the colony formation at each HCS concentration was observed by pre-exposing the cells to both MWR and hyperthermia proceeding culture in HCS. We conclude that MWR and hyperthermia when employed together interact with HCS in a synergistic fashion to inhibit growth of L1210 cells.
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Ottenbreit, M., Inoue, S., Lin, J. et al. 847 THE SYNERGISTIC EFFECT OF MICROWAVES, HYPERTHERMIA AND HYDROCORTISONE SUCCINATE ON L1210 LEUKEMIC CELLS. Pediatr Res 15 (Suppl 4), 583 (1981). https://doi.org/10.1203/00006450-198104001-00872
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DOI: https://doi.org/10.1203/00006450-198104001-00872