Abstract
The histological diagnosis of extra renal Wilm's tumor may be difficult to make. A 7 year old female had an intrabdominal tumor which histologically was undifferentiated and no diagnosis could be reached. Glucose-6-phosphatase as a marker for kidney tissue was assayed. A small fragment of tumor was sonicated in 2 volumes of cacodylate buffer 0.15 M, pH 6.5, then centrifuged at 750 × g for 10 min. Assay for glucose-6-phosphatase was carried out using 0.1 m1 of supernatant extract incubated with 0.1 m1 of 0.15 M glucose-6-phosphate. Incubation was carried out at 37°C for 60 min. Glucose liberated in this reaction was determined by the glucose oxidase method. The enzyme extract from the tumor was found to have glucose-6-phosphatase activity similar to the activity in a control kidney extract. The tumor sample released 1.07 μM, glucose/mg protein/hour, while human kidney extracted hydrolyzed 1.01 μM glucose/mg protein/hour. Blood cells, muscle and ovarian tissues were assayed for glucose-6-phosphatase had no measurable activity. These studies suggested renal origin of the tumor, and subsequent electron microscopy confirmed the diagnosis of Wilm's tumor. Another case of intrarenal tumor was studied with no measurable glucose-6-phosphatase activity, while extract from the same kidney released 1.6 μM/glucose/mg protein/hour. Subsequently the diagnosis of Burkitt lymphoma was made. These data indicate that glucose-6-phosphatase can be used to ascertain the renal origin of intraabdominal tumors.
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Matalon, R., Pang, E., Michals, K. et al. 834 GLUCOSE-6-PHOSPHATASE: A MARKER ENZYME FOR NON-RENAL WILM'S TUMOR. Pediatr Res 15 (Suppl 4), 581 (1981). https://doi.org/10.1203/00006450-198104001-00859
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DOI: https://doi.org/10.1203/00006450-198104001-00859