Abstract
Scanning electron microscope(SEE) is the most suitable method for studying the three-dimensional ultra structure of the renal glomeruli. But it is necessary to compare observations by SEM with those by transmission electron microscope(TEM) simultaneously to exclude various artificial changes which are noticed sometimes on observations by SEM. Renal biopsy specimens were obtained from 13 patients of nephrotic syndrome,aged 5-16 years ( 4 in remission,9 in no remission). All specimens were cut into small cubes, immersed in 2% osmium tetraoxide buffer solution,dehydrated in a siries of alcohol and embedded in styrene-methacrylate resin. After ultra-thin sectioning for TEM,residual surface of blocks were observed by SEM. The observations by SEM and TEE of the same glomeruli from 13 patients with or without proteinuria revealed same findings. 4 cases in remission that had the clear foot processes by TEM showed regular and well arranged terminal processes which looked fern leaf like branching. 9 in no remission had irregularly swollen podocyte corresponding to the amount of proteinuria.
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Fujita, M., Kuronuma, T., Aoyama, R. et al. THE COMBINED SCANNING AND TRANSMISSION ELECTRON MICHOSCOPE STUDY OF NEPHROTIC SYNDROME IN CHILDREN. Pediatr Res 14, 1003 (1980). https://doi.org/10.1203/00006450-198008000-00177
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DOI: https://doi.org/10.1203/00006450-198008000-00177