Abstract
A streptomycin mutant of Escherichia coli strain C94 [07:K1: H-] (C94/sm) representative of the most common capsular type observed in neonatal sepsis and meningitis, and childhood pyelonephritis (PYE) was used to develop a model of PYE. Intraperitoneal (IP) inoculation of normal 21 day old Wistar rats with 107 CFU/ml of C94/sm produced bacteremia persisting for 24 to 72 hours in 80% of the rats. Kidney infections of 102 to 103 CFU/ml were observed in all bacteremic rats for 4 to 14 days. Urine cultures were uniformly negative. Unilateral palpation of the kidney during bacteremia did not appear to have any effect on the degree of infection in normal Wistar rats.
IP inoculation of 107 CFU/ml of C94/sm in congenital unilateral hydronephrotic male Wistar rats produced similar results as above except that the right kidney had a 1-2 log greater bacterial concentration per ml than the left kidney. Palpation of the right kidney during early bacteremia resulted in gross changes in the right kidney evident in less than 72 hours. Bacterial counts of the right kidney exceed the left by 2-4 logs and urines were uniformly positive with > 106 CFU/ml. Experimental PYE in the congenital unilateral hydronephrotic rat appears to be a reproducible model for the study of this disease.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Craft, J., Boineau, F., Daum, R. et al. EXPERIMENTAL PYELONEPHRITIS IN CONGENITAL UNILATERAL HYDRONEPHROSIS IN THE RAT. Pediatr Res 14, 980 (1980). https://doi.org/10.1203/00006450-198008000-00039
Issue Date:
DOI: https://doi.org/10.1203/00006450-198008000-00039