Abstract
We recently obtained a striking increase of the survival rate in very small pre-term infants with sepsis through dayly transfusions of packed PMN (20ml/Kg equal to 0.5 × 1010 cells). In order to increase the availability of PMN concentrates and to reduce the risk of sentization, it would be useful to transfuse repeatedly, in the same patient, PMN collected from the same donor. We, therefore, evaluated the rate of functional decay of packed PMN obtained by leukafiltration and kept at +4C° for 48 hrs, for phagocytosis, candidacidal activity, chemotaxis and spont. & stim. NBT test (for methods see : Ital . J.Pediat. 4:571, 1978). Phagocytosis, candidacidal activity and NBT test remained unchanged at 24 and 48 hrs (104% and 100%, 94% and 93%, 94% and 92% respectively of initial values). Chemotaxis only showed a slight decrease (98% and 73%). The present results show a satisfactory functional stability of PMN collected by leukafiltration, suggesting that the same concentrate may be effectively transfused for three consecutive days.
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Laurenti, F., Balducci, R., Crispino, P. et al. Functional activity of packed polymorphonuclear leukocytes (PMN) obtained by leukafiltration: 85. Pediatr Res 14, 178–179 (1980). https://doi.org/10.1203/00006450-198002000-00112
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DOI: https://doi.org/10.1203/00006450-198002000-00112