Abstract
Recent development of neonatal screening for hypothyroidism with the use T4 measurement in small quantities of serum indicates that in premature babies T4 level frequently lower than 2 S.D. below the mean value is due to RDS or small body weight. To reduce the number of false-positives in T4 screening program for neonatal hypothyroidism low cost screening method of distribution binding ratio of thyroxine/DBR/with TBG and TBPA was prepared. The method is based on electrophoresis of serum sample obtained from U tubes on agarose plates. For direct DBR determination as well indirect determination of endogenous level of T4 and TBG capacity 2 testing doses of radioactive T4 I125/ lug/dl/together with non-radioactive T4/15,30 ug/dl/were added to the 20 ul of examined serum. 200 neonates and 50 premature babies/20 with small body weight below 1700 g and 4 with RDS/ were screened. Besides influence of physical factors on DBR were tested. No essential influence of physical factors on DBR value was proved. However correlation among pH, pO2 of blood and DBR was noticed. In prematures babies with RDS and small body weight DBR values and TBG capacity were lower than in full term newborns.
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Lukas, W., Lewandowski, J. & Halvorsen, S. NEW SCREENING METHOD FOR HYPOTHYROIDISM. Pediatr Res 13, 85 (1979). https://doi.org/10.1203/00006450-197901000-00095
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DOI: https://doi.org/10.1203/00006450-197901000-00095