Abstract
Univ.-Kinderklinik Heidelberg, GFR: Phospholipid patterns and surface activity of neonatal tracheal effluent.- Tracheal aspirates were analyzed in 150 newborn infants when therapeutic suctioning of the endotracheal tube was required. Dynamic surface tension measurements of hystereses (HYS) and minimal surface tension (r min) were performed. Using 2-dimensional TLC and Phosphorus-determination (P), the following phospholipids were identified: Sphingomyelin (SPH), Phosphatidylcholin (PC), Phosphat-idylserin (PS), Phosphatidylinositol (PI), Phosphatidylglycerol(PG) Six patterns characteristic for the infant's clinical condition were found: 1.NORMAL TERM PATTERN: Wide HYS, r min <10 dyn/cm, PC 61 %, PI 10%, PG 10% P,L/S ratio 4.88, PI/PS-ratio 1.59. 2. RDS-EARLY STAGE: Narrow HYS, r min > 20 dyn/cm, PC 41% P. PI 12% P, PG absent, L/S-ratio 1.51, PI/PS-ratio 0.93. 3. RDS-RECOVERY STAGE: Narrow HYS, r min 10-20 dyn/cm, PC 59% P, PI 18% P, PG absent, L/S-ratio 5.5, PI/PS-ratio 3.2. 4. ACCELERATION (preterm infants with chronic prenatal stress like prolonged rupture of membranes): Wide HYS, r min < 10 dyn/cm, PC 62% P,PI 14% P, PC 4% P, L/S-ratio 4.6, PI/PS-ratio 2.8. 5. RETARDATION ( delayed surfactant maturation like maternal diabetes): Narrow HYS, r min > 20 dyn/cm, PC 46 % P, PI 11% P, PG 1.9% P, L/S-ratio 1.48, PI/PS-ratio 0.9. 6. INDUCTION (preterm infants after maternal glucocorticoid administration within 24 and 72 hours before birth): Wide HYS, r min < 15 dyn/cm, PC 59% P, PI 13% P, PG 7% P, L/S-ratio 4.94, PI/PS-ratio 2.51. Tracheal phospholipid composition changes after birth and during RDS. The presence of PG excludes RDS with a specifity of 0.84, its absence proves RDS with a sensivity of 0.94.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Obladen, M., Brendlein, F. & Menzel, T. Phospholipid patterns and surface activity of neonatal tracheal effluent. Pediatr Res 13, 81 (1979). https://doi.org/10.1203/00006450-197901000-00073
Issue Date:
DOI: https://doi.org/10.1203/00006450-197901000-00073