Abstract
Corticosteroids influence intestinal function.Glucocorticoid receptors are present in cytosol of small intestinal mucosa of normal and steroid depleted adult rats.We examined the distribution of cytoplasm ic glucocorticoid receptor proteins in small intesti nal villous and crypt cells using the technique of Weiser to separate cells into 6 fractions.Fraction 1 was highly enriched in villous tip cells and fraction 6 in crypt cells. Alkaline phosphatase,sucrase and 3H-Thymidine incorporation were used to monitor the composition of each fraction.Steroid cytosol receptor activity was measured in each fraction using 3H-dexamethasone and a standard charcoal adsorption technique In normal rats receptor activity was detected in all fractions but was significantly greater in fraction 6 than in fraction 1(p<0.005).These data suggest that cytoplasmic corticosteroid receptors in normal rats have higher binding activity in crypt cells than in villous tip cells. Corticosteroid hormones may influen ce immature crypt cells more than mature villous tip cells in normal rats since glucocorticoid receptor activity is substantially higher in crypt dells.
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Lentze, M., Moxey, P. & Trier, J. Distribution of Glucocorticoid Receptors in Rat Intestinal Epithelium. Pediatr Res 13, 1192 (1979). https://doi.org/10.1203/00006450-197910000-00071
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DOI: https://doi.org/10.1203/00006450-197910000-00071