Abstract
While LPS is the most potent B cell activator in mice, in man induction of B-cell differentiation has been reported only under stringent culture conditions. In 7-day cultures of 10 peripheral blood lymphocytes in 0.2 ml of RPMI containing 10 % FCS in flat-bottomed culture plates, LPS over a wide range of concentrations (0.1 to 250/ug/ml) does not stimulate detectable IgM production while ConA at optimal (6 ug/ml) and suboptimal (0.6 ug/ml) mitogenic concentrations induces synthesis of only low amounts of IgM (250-600 ng/ml). However, when LPS is added to ConA-stimulated cultures a striking dose-dependent enhancement of IgM production, maximum (1200-2100 ng/ml) with 10 ug/ml of LPS is evident. A similar enhancing effect of LPS on IgM production with a mean increase of 100 % was observed also in PWM-stimulated cultures. Studies are now underway to clarify the cellular basis underlying this phenomenon.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vitiello, A., Nospoli, L., Maccario, R. et al. 35 INTERACTION OF LPS AND ConA IN HUMAN LYMPILOCYTE ACTIVATION. I: SYNTERGISTIC EFFECT ON IgM PRODUCTION. Pediatr Res 13, 954 (1979). https://doi.org/10.1203/00006450-197908000-00051
Issue Date:
DOI: https://doi.org/10.1203/00006450-197908000-00051