Abstract
Human peripheral blood lymphocytes (106/ml) from 5 healthy young adults were cultured for 10 days in serum-supplemented RPMI 1640 and stimulated with PWM. Data recording at daily intervals revealed the following kinetic pattern: a) thymidine ia corporation rate reached a maximum (50-100x103 cpm) between days 5 and 7; b) highest percentage of plasmablasts (25-42% was observed on day 7 whereas the number of plasmablasts per culture usually peaked 24 hrs earlier (82-150x103); c) peak rate of IgB release in the supernatant (380-2800 ng/ml/24 hrs) was attained after 6-7 days, whereas the concentration of IgM in the supernatant obviously increased throughout the culture period reaching values of about 1200-8000 ng/ml; d) PFC against SRBC haptenated with PITC peaked on day 7 (144-500/106 cells). A close regular correlation was observed between PFC generation and rate of IgM release despite wide variations in magnitude and kinetics of individual responses. No correlation was found between percentage and absolute number of plasma-blasts. In vitro studies of B cell differentiation in iamunodeficiencies should be expected to give valid information only if the above cited parameters are taken into account following a kinetic approach.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nespoli, L., Vitiello, A., Maccario, R. et al. HUMAN B CEIL DIFFERENTIATION IN VITRO. KINETICS TO THE RESPONSE TO POKEWEED MITOGEN (PWM). Pediatr Res 12, 69 (1978). https://doi.org/10.1203/00006450-197801000-00055
Issue Date:
DOI: https://doi.org/10.1203/00006450-197801000-00055