Abstract
Although eosinophilia is reported in parasitic and other infections the total leukocyte count frequently is not elevated. This suggests a possible inhibitory effect of eostnophiles on granulopoiesis. This study was undertaken to examine the effects of monospecific rabbit anti-mouse anti-eosinophilic sera (AES) on the colony forming ability of Trichinella spiralis infected (30% marrow eos) and normal (2% marrow eos) mice. Marrow from inbred C57 Black mice infected with Trichinella spiral is was cultured in semi-solid media with 1) Human Colony Stimulating Activity (CSA), 2) CSA±AES, 3) CSA±normal rabbit serum (RS), 4) AES alone, and 5) α-media alone. Identical experiments were repeated with normal mice. In infected mice, the number of CFU-C/105 cells cultured with CSA was 72 and with RS, 52. With AES+CSA, the colony number rose to 157 and with AES+RS to 110 (P<0.001 for both). The addition of AES alone or α-media alone produced <5 colonies. These results are in keeping with the fact that mice, unlike the human, lack CSA producing cells in the marrow and thus require exogenous CSA to elicit the effects of AES. The CFU-C promoting effects of AES with CSA did not occur in normal mice. Examination of the colonies by light microscopy revealed neutrophilic series. It thus appears that the effective reduction in eosinophiles and the parallel increase in neutrophilic colonies indicates an inhibitory effect of eostnophiles in maintaining granulopoietic hemeostasis.
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Tebbi, K., Mahmoud, A. & Gross, S. ENHANCING EFFECT OF RABBIT ANTI-MOUSE EOSINOPHIUC SERUM (AES) ON GRANULOPOIETIC COLONY FORMING UNITS IN CULTURE (CFU-C). Pediatr Res 11, 482 (1977). https://doi.org/10.1203/00006450-197704000-00674
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DOI: https://doi.org/10.1203/00006450-197704000-00674