Abstract
Since both maturity and anesthesia are known to produce variations in the response of the cardiovascular(CV)system to drugs, we developed a chronically instrumented puppy model to study the effects of drugs on conscious, developing canines.
Eight puppies 10 to 82 days old were used. Five were less than 17 days old and not yet weaned. Through a median sternototomy and left flank incision, pulsed doppler flow probes(1-8 mm)were placed around the ascending aorta and left renal artery and the wires exteriorized. An infusion of 20% glucose(100cc/kg)was maintained during the operation and the early postoperative period. Puppies not weaned were returned to their mothers after operation.
Three puppies died within 24 hours of operation; 2 died with a ruptured aorta 7 days postoperatively, and 3 are alive 2-3 months after operation. Reproducible blood flow tracings were obtained from flow meters implanted as long as 3 months. In preliminary drug studies(without anesthesia), heart rate(HR) and aortic blood flow were increased more with isoproterenol(I) than with dopamine (D) or dobutamine (Db). In contrast, aortic and renal blood flow were increased more by D and Db than by I and HR was increased equally by all 3 drugs in anesthetized open chest preparations.
This technique permits the study of the CV response of conscious immature animals to drugs and changes of these responses with age.
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Driscoll, D., Lewis, R., Hartley, C. et al. THE CHRONICALLY INSTRUMENTED PUPPY: A NEW RESEARCH TECHNIQUE. Pediatr Res 11, 389 (1977). https://doi.org/10.1203/00006450-197704000-00119
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DOI: https://doi.org/10.1203/00006450-197704000-00119