Abstract
A continuous cell culture line derived from a human lung tumor which has morphological characteristics of type II alveolar epithelial cells was studied for the ability to synthesize and secrete phospholipid. The cells contained 0.213 mg lipld/mg protein of which 41% was phosphatidylcholine (16% of the phosphatidylcholine was dlsaturated). The specific activities of choline kinase and choline phosphotransferase were 381 and 1030 pmoles/min/mg protein, respectively. The cells incorporated choline into phosphatidylcholine (28% of the phosphatldylcholine was disaturated) and secreted phosphatidylcholine into the culture medium (28% of the phosphatidylcholine was disaturated). To study the influence of the adenylate cyclase-cyclic AMP system on these processes, cells were cultured in the presence of 10−3M theophylline. Lipid content and composition were not significantly changed. Choline kinase and choline phosphotransferase specific activities were 172% and 88% of control values, respectively. Choline incorporation into phosphatidylcholine was 209% of the control value (28% of the phosphatidylcholine was disaturated). Acute stimulation of phospholipid secretion was not produced by theophylline addition. Homogeneous cell cultures can be used to study the effects of hormones on phospholipid biosynthesis and secretion.
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Shapiro, D., Rooney, S., Nardone, L. et al. PHOSPHOLIPID BIOSYNTHESIS AND SECRETION IN CULTURES OF TYPE II ALVEOLAR EPITHELIAL TUMOR CELLS: THE EFFECT OF THEOPHYLLINE. Pediatr Res 11, 579 (1977). https://doi.org/10.1203/00006450-197704000-01254
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DOI: https://doi.org/10.1203/00006450-197704000-01254