Abstract
A sensitive radioimmunoassay (RIA) for plasma AVP has been developed. The extraction from plasma following the procedure described by G.L.Robertson allows a recovery of 70% of AVP. A highly specific antibody has been obtained in rabbits immunized with Lysine VP coupled to thyroglobulin. The sensitivity of the assay is 1 pg/ml. The half life of the hormone measured after injection of AVP in diabetes insipidus (DI) is 4.5 min (n=2), in nephrogenic DI 6 min (n=1) and in renal insufficiency after bilateral nephrectomy 6 min (n=1). Base line values in normal children range between ≤ 1 pg/ml to 4 pg/ml. During dehydration AVP increases in plasma. Preliminary results in children from 6 to 14 years indicate that 285 mosm/L is the critical value above which AVP is secreted in proportion with plasma osmolality. In DI of several etiologies, 10 patients exhibited a small increase of plasma AVP during dehydration. Correlation with plasma osmolality indicates that this elevation occurs for values of osmolality which are higher than in normal children. Therefore many patients with DI may retain some capacity to secrete AVP but this secretion is inadequate in regards to plasma hyperosmolality.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Czernichow, P., Basmaciogullari, A. ARGININE VASOPRESSIN (AVP) RELEASE DURING SHORT DEHYDRATION TEST IN CHILDREN. Pediatr Res 9, 667 (1975). https://doi.org/10.1203/00006450-197508000-00019
Issue Date:
DOI: https://doi.org/10.1203/00006450-197508000-00019