Abstract
11 puppies of 2–3 weeks of age and either sex were subjected to a standardized haemorrhagic shock. Aldosterone (A), corticosterone (B), cortisol (F), deoxycorticosterone (DOC), progesterone (P) and 17 α-OH-progesterone (17 α) were determined simultaneously in 250 ul of peripheral plasma taken at constant dates before, during and after blood withdrawal and re-infusion. Our method included CH2Cl2 extraction, separation by automated Sephadex LH-20 multi-column chromatography and quantitation of each steroid by radioimmunoassay.
During hypovolaemia, plasma levels of the main mineralocorticoid (A) and its precursors (P,DOC,B) increase much more than those of the main glucocorticoid (F) and its precursor (17α). These and additional data on electrolyte and glucose levels reflect the key role of the adrenal cortex in maintaining homoeostasis during shock in the early life period.
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Sippell, W., Golder, W., Hahn, H. et al. SIMULTANEOUS DETERMINATION OF SIX PLASMA CORTICOSTEROIDS IN PUPPIES DURING STANDARDIZED HAEMORRHAGIC SHOCK. Pediatr Res 9, 674 (1975). https://doi.org/10.1203/00006450-197508000-00058
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DOI: https://doi.org/10.1203/00006450-197508000-00058