Abstract
Extract: The retrograde perfusion assay of Mangos for the sodium reabsorption inhibitory effect in sweat and mixed saliva from patients with cystic fibrosis (CF) was modified by (1) using the second parotid gland of the rat as a control, (2) carefully controlling the quantity of saliva perfused into the rat parotid gland so that the ratio of milliliters perfused to gram dry gland weight fell between 1.8 and 2.8, and (3) reporting the inhibitory effect as percentage of inhibition of the rate of sodium reabsorption (RNa) in tne perfused gland calculated from the difference between the RNA'S in the two glands. Using this modified assay we were able to confirm that significant differences exist in the percentage of inhibition of RNa caused by CF and normal mixed saliva (47.0 ± 24.8 and 18.0 ± 8.3, mean ± SD). In addition, similar significant differences in the percentage of inhibition of RNa by submandibular gland secretions (39.7 ± 13.5 and 16.0 ± 11.8), sublingual gland secretions (39.5 ± 6.0 and 19.7 ± 14.3), and submucosal gland secretions (34.0 ± 11.7 and 21.7 ± 12.7) between CF and normal subjects were observed. However, parotid gland secretions from CF subjects showed no increased inhibitory effect (12.5 ± 7.9 and 12.5 ± 6.6). These findings demonstrate that the sodium reabsorption inhibitory effect is produced by the secretions from some but not all salivary glands of patients with CF.
Speculation: These results indicate that the substance or substances responsible for the sodium reabsorption inhibitory effect of CF secretions is produced by cells present in submandibular, sublingual, and submucosal glands of patients with CF. Because mucus-producing cells are abundant in submandibular, sublingual, and submucosal glands, but are sparse in parotid glands (1) we suggest, as have Johansen et al. (4), that the inhibitory effect is in some way related to the mucous secretory product of the cystic fibrosis glands.
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Taylor, A., Mayo, J., Boat, T. et al. Standardized Assay for the Sodium Reabsorption Inhibitory Effect and Studies of Its Salivary Gland Distribution in Patients with Cystic Fibrosis. Pediatr Res 8, 861–865 (1974). https://doi.org/10.1203/00006450-197411000-00001
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DOI: https://doi.org/10.1203/00006450-197411000-00001
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