Abstract
In previous communications we have demonstrated the presence of cryoprecipitable complexes of antigen and specific antibody in the sera of patients and experimental animals with immune complex disease. Recently we have reported localization of renal tubular epithelial antigen (RTE) and specific antibody in the glomeruli of a patient with sickle cell disease and immune complex nephritis and demonstrated an autologous immune complex pathogenesis for the renal lesion. This study describes the isolation of cryoprecipitable complexes of RTE and anti-RTE in this disease. Cryoprecipitates were isolated from the serum as previously described. The cryoprecipitate was dissolved in 3MKI at 37°C and fractions eluted from Sepharose 6B and Sephadex G200 column with 0.2M acetate buffer pH 5. The fractions obtained contained IgG antibody to RTE as demonstrated by immunofluorescent and absorption studies; immunoreactive RTE antigen and IgM antibody to RTE as well as to IgG. No other proteins were detected. These studies support the hypothesis that RTE-anti-RTE complexes are of immunopathogenic significance in the autologous immune complex nephropathy associated with sickle cell disease.
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Strauss, J., Koss, M., Pardo, V. et al. CRYOPRECIPITABLE PATHOGENIC IMMUNE COMPLEXES IN THE NEPHROPATHY ASSOCIATED WITH SICKLE CELL DISEASE. Pediatr Res 8, 461 (1974). https://doi.org/10.1203/00006450-197404000-00727
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DOI: https://doi.org/10.1203/00006450-197404000-00727