Abstract
Basophils from certain allergic subjects will secrete histamine when exposed to appropriate antigens; after repeated washings this reactivity is lost. To examine the possibility that washing removes a membrane-bound factor other than IgE necessary for antigenic histamine release, allogeneic unreactive cells from allergic donors were exposed to washes from reactive cells then challenged with antigen. Histamine release was measured by the usual methods (J.E.M. 120: 507, 1964). Reactive leukocytes were separated in Dextran, washed twice in Tris BSA buffer (pH 7.4) and the washes saved. Unreactive cells were similarly separated, washed, then incubated in Tris BSA buffers with added Ca++ and Mg++ for one hour at 37°C., then resuspended with fresh Tris BSA Ca++ and Mg++ buffer for ragweed antigen challenge. Washes from two or four reactive donors were capable of inducing non-reactive cells to release 62-100% total histamine on antigen challenge; no more than 10% total histamine was released from incubated cells not exposed to antigen. Allogeneic cells from normal non-allergic donors could not be induced to release histamine. The facilitating ability of the washes was not destroyed at 56°C. for one hour and was non-dializable. We concluded that leukocytes capable of antigenic histamine release have a protein on their surface which is not IgE and which is necessary for antigenic histamine release.
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Eggleston, P., Eggleston, A. & Komp, M. FACILITATION OF ANTIGENIC LEUKOCYTE HISTAMINE RELEASE BY A SOLUBLE FACTOR. Pediatr Res 8, 412 (1974). https://doi.org/10.1203/00006450-197404000-00432
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DOI: https://doi.org/10.1203/00006450-197404000-00432