Abstract
Urine from patients with generalized congenital lipodystrophy has been fractionated by precipitation of the protein with benzoic acid succeeded by Sephadex gel filtration. A fraction with mol.wt. in the range of 1,500 was found to have metabolic activity in intact rabbits and mice. In acute experiments there was observed an increase of serum triglycerides only. Following 20 days of injection of the lipodystrophic fraction (LDF) a lipodystrophic state was induced in the animals, with increase of BMR, and decrease of body weight in spite of a 40% increase of food consumption. The fat depots disappeared, blood glucose increased, and an insulin resistance was developed. Urine obtained from the injected rabbits induced similar changes in mice. Following injection of LDF in infantile rats there was observed a growth of the gonades, and a significant increase of serum somatomedin. In hypophysectomized rats there were observed no growth of the tibial width or of the gonades. It is concluded that the LDF probably contained hypothalamic releasing hormones, which also may be responsible for the development of the disease.
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Foss, I., Trygstad, O. PREPARATION OF A URINARY LIPODYSTROPHIC FRACTION. Pediatr Res 8, 131 (1974). https://doi.org/10.1203/00006450-197402000-00033
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DOI: https://doi.org/10.1203/00006450-197402000-00033