Abstract
One μg of synthetic LH-RH(Beckman) in 5 μ1 of normal saline was administered subcutaneously to male (N=14) and female(N=9) rat pups between 10 and 20 hrs. after birth. Control animals (males, N=13; females, N=9) received normal saline. Twenty min. after injection the animals were sacrificed by decapitation. LH levels were determined in serum and whole pituitary glands by radioimmunoassay. LH data are expressed in terms of NIAMDD-Rat LH-RP-1. In males pituitary LH content was similar in control (0.595±.364(SD) μg/pituitary) and LH-RH injected animals (0.518±.135 μg). In females pituitary LH content was lower in LH-RH treated rats than in control animals (0.527±.110 μg, 0.725±.184 μg, p<0.02).
Serum LH concentrations (ng/ml) in female control pups were significantly higher than in male control pups (p<0.01). Serum LH concentrations were significantly higher in male and female pups who had received LH-RH than in animals injected with saline:
These data indicate that LH-RH stimulates the secretion of LH in male and female newborn rat pups.
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Shapiro, B., Root, A., Goldman, A. et al. EFFECT OF LUTEINIZING HORMONE RELEASING HORMONE(LH-RH)UPON THE SECRETION OF LH IN NEWBORN RATS. Pediatr Res 8, 375 (1974). https://doi.org/10.1203/00006450-197404000-00209
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DOI: https://doi.org/10.1203/00006450-197404000-00209