Abstract
Hydrogen ion secretion by epithelial structures can be mediated by a cation exchange mechanism as in the gills of some fish or by an ‘active’, pump-mediated specific process. Experiments were made to test the cation exchange mechanism in isolated amphibian skins by substitution of Na+ by other ions in the bathing fluids, by inhibiting Na+ influx with amiloride, or by stimulating it with Pitressin. Chambers of the Ussing type were used and the H+ secretion rate was monitored by the pH stat method. In the frog skin, suppression of Na+ influx by amiloride as well as its stimulation by Pitressin had no effect on H+ secretion rates. Again, Na+ substitution by Mg+ did not influence H+ secretion. However, experiments done in toad skin showed that H+ secretion increased after stimulation with Pitres sin and decreased or sometimes was suppressed when amiloride was used or when Na+ was substituted, suggesting that in this last preparation, some part of the total H+ secretion is linked to Na+ influx. In the isolated frog skin this mechanism seems not to be operative.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Menano, H., Emilio, M. The effect of amiloride in H+ secretion in the isolated amphibian skin.. Pediatr Res 8, 900 (1974). https://doi.org/10.1203/00006450-197411000-00035
Issue Date:
DOI: https://doi.org/10.1203/00006450-197411000-00035