Abstract
The existence of specific inhibitors of erythropoiesis (EIF) has been suggested by several authors. The demonstration of this inhibitor, suggests that both activators (ESF) and inhibitors (EIF) participate in the normal regulation of erythropoiesis. In this study the presence of EIF in urine and neonatal plasma has been investigated.
Urine from normal healthy persons, from patients with aplastic anemia, and from patients with sever anemia due to chronic renal failure was investigated. The urine were passed through a Sephadex coloumn, and the different fractions were tested for stimulatory and inhibitory effect of erythropoiesis. Plasma from normal newborn babies was withdrawn on the first 4–6 days of life. Plasma from newborns with hyperbilirubinemia but normal hemoglobin levels was also used for the plasma studies. Erythropoiesis in exhypoxic polycythemic mice was either stimulated by erythropoietin injections or by a second hypoxic period. Saline and test material were given simultaneously with erythropoietin or before and after the second hypoxic period. The effect was measured as 59Fe uptake in red blood cells.
A marked inhibitory effect was found in the urine from normal healthy persons and from patients with aplastic anemia. In patients with chronic uremia a slight inhibitory effect and no active erythropoietin was found. The mice given neonatal plasma showed a marked reduction in iron uptake when the plasma was withdrawn between the 4th and 14th days of life.
The mode of action and the physiological role of EIF are unknown. EIF may be related to chalones> This is supported by the finding that EIF has the same molecular weight as reported for the chalones.
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Halnorsen, S., Lindemann, R. & Skjaelaaen, P. 52. Erythropoietic inhibitors in plasma and urine. Pediatr Res 5, 94 (1971). https://doi.org/10.1203/00006450-197102000-00057
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DOI: https://doi.org/10.1203/00006450-197102000-00057