Abstract
Antibodies to the O antigen of infecting Escherichin coli strains have been studied in children with UTI. No relation between levels of O anitbodies and protection against UTI has yet been ascertained in humans. We wanted to study the appearance and possible significance of capsular antibodies.
By direct bacterial agglytination with appropriate controls antibodies to K antigens could be demonstrated in a few patients iwht UTI. For further investigation a few well characterized K antigens of the acid polysaccharide type were isolated from E coli strains by preparative sone electrophoresis. These antigens were employed to study with the passive hemagglytination technique the K antibody response in rabbits immunized with E. coli. A marked K and O antibody response mainly consisting of reduction sensitive 19 S antibodies was observed after a single injection of bacteria. After a booster dose a secondary type response including increased titres of 7 S as well as 19 S antibodies was obtained against both K and O antigens. The protective effect of these rabbit antibodies was evaluated in mouse protection experiments employing for challenge the homologous E. coli strain as well as two serologically related strins. These experiments illustrated the serological specificity of protective antibodies. The obtained data may help in the evaluation of the possible significance of the K antibodies appearing in patients with UTI.
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Kaijser, B., Borssén, R., å. hanson, L. et al. 30. Capsular antibodies to Escherichia coli in relation to urinary trait infectious (UTI). Pediatr Res 5, 89 (1971). https://doi.org/10.1203/00006450-197102000-00035
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DOI: https://doi.org/10.1203/00006450-197102000-00035