Abstract
Human chromosomes are preferentially eliminatd from man/mouse somatic hybrid cells. Two groups of works obtained viable hybrids by mixing human cells with a murine cell line lacking thymidine kinase and growing the mixture of cells in a selective medium in which cell survival required the presence of this enzyme. The hybrid cells usually contained a single human submetacentric chromosome, whose size and shape suggested that it was a memeber of the E-group, either chromosome 17 or 18. This chromosome presumably carries the human thymidine kinase locus.
Chromosomes of the E-group can be readily identified in human cells by their distinctive quinacrine-fluorescence patterns. By applying this technique to metaphase figures from one of the hybrid cell lines studied by Migeon and Miller (Science 162, 1005, 1968), we have found that the submetacentric chromosome which is present has the characteristic quinacrine-fluorescence pattern of a human chromosome 17.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Miller, O., Allderdice, P., Miller, D. et al. Assignment of human thymidine kinase gene locus to chromosome 17 by identification of its distinctive quinacrine-fluorescence in man/mouse somatic hybrid cells. Pediatr Res 5, 424 (1971). https://doi.org/10.1203/00006450-197108000-00220
Issue Date:
DOI: https://doi.org/10.1203/00006450-197108000-00220