Abstract
Peripheral blood samples and fibroblast cultures from 5 children with Fanconi's anemia and 5 contorl were irradiated with 10 and 100 rads; other controls were non-irradiated with 10 and 100 rads; other controls were non-irradiated duplicate cultures. Blood cultures were harvested at 52 and 72 hours. Fibroblast cultures were irradiated 24 hours before fixation. Radiation break frequencies were calcultaed by subtracting figures for non-irradiated samples from those fro irradiated samples. The number of chromosome-type breaks per cell per rad in irradiated controls was 0.0025 ± 0.0005 (52 hour cultures) and 0.0017 ± 0.005 (72 hours cultures) in lymphocytes, and 0.0059 ± 0.0016 in fibroblasts, and in irradiated cells from Fanconi's anemia was 0.0103 ± 0.0016 (52 hour cultures) and 0.0074 ± 0.0036 (72 hour cultures) in lymphocytes, and 0.0103 ± 0.0034 in fibroblasts. The number of dicentrics and rings per cell was significantly greater in the Fanconi's anemia samples than controls at 100 rads. We concluded that, in-vitro, chromosomes of cells from children with Fanconi's anemia were significantly more radiosensitive than those of controls (P < 0.01).
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Higurashi, M., Conen, P. & Laski, B. In-vitro chromosomal radiosensitivity in Fanconi's anemia. Pediatr Res 5, 407 (1971). https://doi.org/10.1203/00006450-197108000-00151
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DOI: https://doi.org/10.1203/00006450-197108000-00151